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By Raymond L. Rodriguez, David T. Denhardt

ISBN-10: 0409900427

ISBN-13: 9780409900422

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1984 Marsh et al. 1984 Marsh et al. 1984 Hagan and Warren 1982 Honigman and Oppenheim 1981 Hennecke et al. 1982 Enger-Valk et al. 1980 Norrander et al. 5 pMBl pKLl bc Genetic Markers Size (kb) Replicon Continued Plasmid TABLE 1-2 Hindlll, Pstl, BamHl, Sali, EcoRl, Smal EcoRl BamHl, Hpal Hindlll Sacl, EcoRl, Smal, Hindlll, BamHl, Smal Clal, Bgll Pstl Hindll, Hpal Xmal, EcoRl Hindlll, Bell, Xmal, Smal, EcoRl EcoRl Pstl, Sail, Accl, BamHl, Hindlll, EcoRl Accl, Xbal, Sali, BamHl, Hincll, EcoRl EcoRl Sali Known Unique Restriction Sites3 Churchward et al.

It is not surprising, therefore, that it would also serve as one of the first molecular cloning vectors. T h e life cycle and genetics of this important bacterial virus have been extensively reviewed elsewhere (Hendrix et al. 1983). F u r t h e r m o r e , the entire nucleotide sequence of the wild-type lambda g e n o m e has recently been determined (Daniels et al. 1983). T h e results of these and other studies indicate that the lambda phage particles are composed of approximately equal parts of protein and D N A .

And Tomizawa, J. (1983) Cell 32, 119-129. E. (1983) Proc. Natl. Acad. Sei. USA 80, 1797-1801. D. (1982) Nature 299, 312-316. D. (1983) Proc. Natl. Acad. Sei. USA 80, 2447-2451. , and Gustaffson, P. (1983) Gene 23, 105-115. J. (1983) Mol. Gen. Genet. 192, 444-451. , and Falkow, S. (1975) Mol. Gen. Genet. 142, 239-249. , and Bolivar, F. (1980) Gene 9, 287-305. J. (1982) Proc. Natl. Acad. Sei. USA 79, 7253-7257. , and Tomizama, J. (1983) Proc. Natl. Acad. Sei. USA 80, 3232-3236. , and Broome-Smith (1986) Gene 41, 337-342.

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Vectors. A Survey of Molecular Cloning Vectors and their Uses by Raymond L. Rodriguez, David T. Denhardt


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