Get Vectors. A Survey of Molecular Cloning Vectors and their PDF

By Raymond L. Rodriguez, David T. Denhardt

ISBN-10: 0409900427

ISBN-13: 9780409900422

Show description

Read or Download Vectors. A Survey of Molecular Cloning Vectors and their Uses PDF

Similar cell biology books

Download e-book for iPad: Current Protocols Essential Laboratory Techniques by Sean R. Gallagher, Emily A. Wiley

The most recent name from the acclaimed present Protocols sequence, present Protocols crucial Laboratory strategies, 2e presents the hot researcher with the talents and figuring out of the basic laboratory systems essential to run winning experiments, clear up difficulties, and develop into a efficient member of the trendy existence technology laboratory.

Melanoma Development: Molecular Biology, Genetics and by Anja Bosserhoff, Luigi Strizzi (auth.), Anja Bosserhoff PDF

The e-book presents entire insights into molecular adjustments in malignant cancer. the final mechanisms of cancer progress and improvement are defined, in addition to new examine findings. Our present wisdom at the molecules concerned with phone transformation and tumor development will quickly result in subtle, detailed treatments.

David M. Glover's Gene Cloning: The Mechanics of DNA Manipulation PDF

This booklet was once originallyconceived within the shape ofa moment version ofa quantity released in 1980 in Chapman and Hall's 'OutllneStudies in Biology' sequence and entitled Genetic Engineering - Cloning DNA. It very speedily grew to become obvious that with the influence ofrecombinant DNA concepts being feIt in such a lot of components ofblology, it used to be going to be difficultifnotimpossible to keepthe bookwithin the distance confines of those little monographs.

John Haycock, Arti Ahluwalia, J. Malcolm Wilkinson's Cellular In Vitro Testing: Methods and Protocols PDF

Growing to be cells in 2nd less than static stipulations has lengthy been the ultimate of mobilephone tradition, regardless of this system no longer being consultant of the advanced in vivo setting. using animal types additionally has transparent moral and clinical barriers, and more and more the 3Rs (replacement, refinement, aid) relating to animal types are being built-in into the modern day medical perform.

Additional info for Vectors. A Survey of Molecular Cloning Vectors and their Uses

Example text

1984 Marsh et al. 1984 Marsh et al. 1984 Hagan and Warren 1982 Honigman and Oppenheim 1981 Hennecke et al. 1982 Enger-Valk et al. 1980 Norrander et al. 5 pMBl pKLl bc Genetic Markers Size (kb) Replicon Continued Plasmid TABLE 1-2 Hindlll, Pstl, BamHl, Sali, EcoRl, Smal EcoRl BamHl, Hpal Hindlll Sacl, EcoRl, Smal, Hindlll, BamHl, Smal Clal, Bgll Pstl Hindll, Hpal Xmal, EcoRl Hindlll, Bell, Xmal, Smal, EcoRl EcoRl Pstl, Sail, Accl, BamHl, Hindlll, EcoRl Accl, Xbal, Sali, BamHl, Hincll, EcoRl EcoRl Sali Known Unique Restriction Sites3 Churchward et al.

It is not surprising, therefore, that it would also serve as one of the first molecular cloning vectors. T h e life cycle and genetics of this important bacterial virus have been extensively reviewed elsewhere (Hendrix et al. 1983). F u r t h e r m o r e , the entire nucleotide sequence of the wild-type lambda g e n o m e has recently been determined (Daniels et al. 1983). T h e results of these and other studies indicate that the lambda phage particles are composed of approximately equal parts of protein and D N A .

And Tomizawa, J. (1983) Cell 32, 119-129. E. (1983) Proc. Natl. Acad. Sei. USA 80, 1797-1801. D. (1982) Nature 299, 312-316. D. (1983) Proc. Natl. Acad. Sei. USA 80, 2447-2451. , and Gustaffson, P. (1983) Gene 23, 105-115. J. (1983) Mol. Gen. Genet. 192, 444-451. , and Falkow, S. (1975) Mol. Gen. Genet. 142, 239-249. , and Bolivar, F. (1980) Gene 9, 287-305. J. (1982) Proc. Natl. Acad. Sei. USA 79, 7253-7257. , and Tomizama, J. (1983) Proc. Natl. Acad. Sei. USA 80, 3232-3236. , and Broome-Smith (1986) Gene 41, 337-342.

Download PDF sample

Vectors. A Survey of Molecular Cloning Vectors and their Uses by Raymond L. Rodriguez, David T. Denhardt

by Ronald

Rated 4.85 of 5 – based on 50 votes