By N. J. Chinoy, I. C. Dave, Y. D. Singh, O. P. Saxena, A. V. Vyas (auth.), N. J. Chinoy, I. C. Dave, Y. D. Singh, O. P. Saxena, A. V. Vyas (eds.)
There is a paucity of knowledge at the dynamics of Ascorbic Acid (AA) turnover relating to germination, metabolism, progress, differentiation and improvement of a plant and in these present process pressure of varied forms. in presowing remedy of seeds and so forth. The turnover of AA performs a big position through the juvenile section of development of a plant and has an important concerning its next progress, improvement and maturation. The worthwhile influence of presowing remedy of seed with Ascorbic Acid (AA) + H2 O highlights the validity of the AA-nucleic acid 2 protein metabolism inspiration of development and improvement of plan ts. in the course of the process the final 30 years, paintings has been undertaken through the writer and his collaborators at the meta bolic drifts of regulatory ingredients in the course of juvenile, vegetative, reproductive and senescent levels. an important of those development regulatory components was once discovered to be Ascorbic Acid. The dynamiC position of AA turnover is published through its keep an eye on of charges of metabolic approaches in addition to these of enzymic reactions which paves how one can "New Genetics".
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There's a paucity of data at the dynamics of Ascorbic Acid (AA) turnover with regards to germination, metabolism, progress, differentiation and improvement of a plant and in these present process tension of assorted forms. in presowing remedy of seeds and so forth. The turnover of AA performs an incredible function through the juvenile part of development of a plant and has an important touching on its next progress, improvement and maturation.
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Measurement of intensity of histochemical reaction After the histochemical processing, the intensity of the staining could be measured by a simple, cytophoto~electro meter devised in the author's laboratory (Chinoy et a1.. ,- liI6). A pointolite microscope lamp (A) PJoduces a beam of light which after passing through the condenser (B) and a blue filter illuminates the preparation on the slide kept on the stage (C). This light passes through the obje- ctive (E) and the prism (F) and emerges through the eye piece (G), which protrudes out of the dark chamber and is reflected by the mirror (H) on the platform (I) with a very small aperture below which is a photo-electric cell (JPhotronic Cell, Weston, Model S.
68. Inagaki C, Fukuba H, Kukai M, Toyosato S 1955, Nippon Nogeikagaku Kaishi 29: 416. 69. Inczedy J 1961, Acta Chim Acad Sci Hung 27: 185. 70. Jackson SF, Chicester CO, Joslyn MA 1960, Food Res 25: 484. 71. m Biophys Acta 653: 423. 26 72. Janecke H 1954, Deut Lebensm Rundschau 50: 65. 73. Jonaitis H, Krisciun1ene B 1962, L1etuvas Fiz Rinkinys TSR Moks1u Akad,'Aukstosios Motyk1os 2: 409. 74. Kamiya S 1960, Nippon Nogeikagaku Kaishi 34: 8 and 13. 75. Kamiya S, Nakabayashi T 1957, Bitamin 13: 250 and 384.
The tissues were washed with three changes of reagent B for 15 minutes in order to remove the excess of silver nitrate. They were then dehydrated using t-buty1 alcohol (TBA) series, embedded 44 in paraffia and mierotomed. The sections were deparaffinated in absolute xylol and mounted in Canada balsam. Black. brownish-black or tan coloured granules or colloidal deposits of silver indicated the presence of AA. The above mentioned modified method was found to be very specific for AA as it was fixed before it had a chance to be displaced from its original position in situ.
The Role of Ascorbic Acid in Growth, Differentiation and Metabolism of Plants by N. J. Chinoy, I. C. Dave, Y. D. Singh, O. P. Saxena, A. V. Vyas (auth.), N. J. Chinoy, I. C. Dave, Y. D. Singh, O. P. Saxena, A. V. Vyas (eds.)