By D Hoessli
This publication specializes in GPI-anchored molecules in biomembranes, their association, biosynthesis, functionality as receptors and use as engineered molecules. an intensive bankruptcy can also be dedicated to discussing the buildings of microbial GPI-anchored molecules and bioactivities on mammalian host cellphone membranes. The experiences of this ebook are meant to express the up to date view of ways GPI-anchored biomolecules engage with and serve as in membranes.
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Additional info for GPI-Anchored Membrane Proteins and Carbohydrates (Molecular Biology Intelligence Unit)
Takami N, Oda K, Ikehara Y. Aberrant processing of alkaline phosphatase precursor caused by blocking the synthesis of glycosylphosphatidylinositol. J Biol Chem 1992; 267:1042-1047. 78. Delahunty, MD, Stafford, FJ, Yuan, LC, et al. Uncleaved signals for glycosylphosphatidylinositol anchoring cause retention of precursor proteins in the endoplasmic reticulum. J Biol Chem 1993; 268:12017-12027. 79. Field MC, Moran P, Li W et al. Retention and degradation of proteins containing an uncleaved glycosylphosphatidylinositol signal.
In earlier reports, several other compounds were identified that inhibited GPI biosynthesis. Although these compounds proved useful in elucidating the steps involved in GPI biosynthesis, they did not distinguish between the mammalian and parasite pathways and/ or were relatively unspecific inhibitors. 44,76,77 However, since mannosamine, 2-fluoro-2-deoxyglucose and amphomycin also affect N-glycosylation, they cannot be used specifically against the GPI biosynthetic pathway. 31,42 Attachment of GPI to Protein GPI anchors are attached to select proteins via the action of an ER enzyme, presumed to be a transamidase (GPI: protein transamidase, or GPT).
Kodukula K, Amthauer R, Cines D et al. Biosynthesis of phosphatidylinositol-glycan (PI-G)anchored membrane proteins in cell-free systems: PI-G is an obligatory cosubstrate for COOH-terminal processing of nascent proteins. Proc Natl Acad Sci USA 1992; 89:4982-4985. 84. Doering TL, Schekman R. Glycosyl-phosphatidylinositol anchor attachment in a yeast in vitro system. Biochem J 1997; 328,669-675. 85. Vidugiriene J, Menon AK. Soluble constituents of the ER lumen are required for GPI anchoring of a model protein.
GPI-Anchored Membrane Proteins and Carbohydrates (Molecular Biology Intelligence Unit) by D Hoessli