Read e-book online Gene Isolation and Mapping Protocols PDF

By Jacqueline Boultwood

ISBN-10: 0896034828

ISBN-13: 9780896034822

An exceptional number of the entire latest suggestions for gene isolation and mapping, together with the newest equipment for gene characterization utilizing database analyses. This number of completely confirmed recipes additionally comprises chapters for the computational research of novel cDNA sequences with up to date info on easy series research, series similarity searches, exon detection and similarity searches, and the prediction of gene functionality. Its cutting-edge equipment represent fundamental instruments for all scientists engaged within the look for particular sickness genes, or within the normal development of the human genome undertaking.

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A. (1985) J. Mol. BioI. 183,611. 9. F. E. (1969) J. Chem. Phys. 51, 635. 10. L. (1985) Cooperativity Theory in Biochemistry (Springer, New York). 11. Oosawa, F. and Asakura, S. (1975) Thermodynamics of the Polymerization of Protein (Academic, New York). 12. L. W. (1982) Int. Rev. Cytol. 78, 1. 13. B. (1983) J. Cell BioI. 97, 542. 2 Attached Single-Stranded PolYlIler In the remainder of the book, except for Chapters 5 and 6 and Section 24, we shall treat linear polymers formally as if they consist of a single strand only, as in Fig.

Independent Aggregate Attached to a Surface An independent linear polymer or aggregate with one end attached to a surface is a very important special case in cell biology. The polymer is immersed in solvent as before, but it is no longer free to rotate or translate. These degrees of freedom for a free polymer become additional vibrational degrees offreedom for an attached polymer. The partition function QN for an attached aggregate of size N (Section 5) does not include a factor V. Hence, Eq. 8), for Y, applies to this case without modification.

10, pp. 6-12. In summary, Ql has contributions of the following types: (a) Translation and external rotation ofthe single solute molecule (monomer), just as for a single solute molecule in the gas phase. The translational partition function is proportional to V; hence Ql is proportional to V. (b) Internal vibration (and rotation) within the solute molecule, including any perturbations caused by the solvent molecules surrounding the solute molecule. (c) Intermolecular interactions between the solute molecule and the surrounding solvent molecules.

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Gene Isolation and Mapping Protocols by Jacqueline Boultwood

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