By Zbigniew Darzynkiewicz, J. Paul Robinson and Harry A. Crissman (Eds.)
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S. , and Krishan, A. Biochem. Pharmacol. 39, 723-728. ,Hamilton, T. , Perillo, J. , and Young, R. C. J . Clin. Oncol. 5, 641. ,and Krishan, A. Biochem. Pharmacol. 45, 743-75 1. , and Kishan, A. Cancer Chemorher. Pharmacol. 31,431-441. Skovsgaard, T. Cancer Res. 38, 1785-1791. , Samy, T. S. A. , Wellham, L. , Duncan, R. , Anac, S. , Ardalan, B.. and Benedetto, P. W. Cancer Chemorher. Pharmacol. 31,423-430. , and Sakurai, Y. Cancer Res. 41, 1967-1972. CHAPTER 3 Glutathione and Cellular Resistance to Anti-Cancer Drugs David Hedley and Sue Chow Ontario Cancer Institute Princess Margaret Hospital Toronto, Ontario M4X 1K9 Canada I.
Materials A. Stains for Glutathione Monochloro- and monobromobimane: Both of the bimanes are obtainable from Calbiochem or Molecular Probes. Make up 4 mM solutions in 100% ethanol, and store refrigerated or at - 20°C. Solutions are stable for several weeks. Mercury orange: Obtainable from Sigma. Stock solution is 100 p M mercury orange in 100% acetone. This is stable for at least several weeks when stored at - 20°C. Staining solution is 40 pM in acetone, with 5% distilled water added prior to use.
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Flow Cytometry by Zbigniew Darzynkiewicz, J. Paul Robinson and Harry A. Crissman (Eds.)