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By Zbigniew Darzynkiewicz, J. Paul Robinson and Harry A. Crissman (Eds.)

ISBN-10: 0125641435

ISBN-13: 9780125641432

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S. , and Krishan, A. Biochem. Pharmacol. 39, 723-728. ,Hamilton, T. , Perillo, J. , and Young, R. C. J . Clin. Oncol. 5, 641. ,and Krishan, A. Biochem. Pharmacol. 45, 743-75 1. , and Kishan, A. Cancer Chemorher. Pharmacol. 31,431-441. Skovsgaard, T. Cancer Res. 38, 1785-1791. , Samy, T. S. A. , Wellham, L. , Duncan, R. , Anac, S. , Ardalan, B.. and Benedetto, P. W. Cancer Chemorher. Pharmacol. 31,423-430. , and Sakurai, Y. Cancer Res. 41, 1967-1972. CHAPTER 3 Glutathione and Cellular Resistance to Anti-Cancer Drugs David Hedley and Sue Chow Ontario Cancer Institute Princess Margaret Hospital Toronto, Ontario M4X 1K9 Canada I.

Materials A. Stains for Glutathione Monochloro- and monobromobimane: Both of the bimanes are obtainable from Calbiochem or Molecular Probes. Make up 4 mM solutions in 100% ethanol, and store refrigerated or at - 20°C. Solutions are stable for several weeks. Mercury orange: Obtainable from Sigma. Stock solution is 100 p M mercury orange in 100% acetone. This is stable for at least several weeks when stored at - 20°C. Staining solution is 40 pM in acetone, with 5% distilled water added prior to use.

39, 471-479. , and Hittelman, W. N. (1988). Exp. Cell Res. 174, 156-167. Bohr, V. , Smith, C. , Okumuto, D. , and Hanawalt, P. C. (1985). ) 40, 359-369. , and Howard-Flanders, P. (1964). Proc. Nut/. Acad. Sci. S . A . 51, 293-300. , and Hanawalt, P. C. (1967). Science 158, 663-664. , and Gray, J. W. (1988). Cytometry 9, 631-635. 1. , Pallavicini. M.. and Gray, J. W. (1983). Proc. Narl. Acad. Sci. S . A . 80, 5573-5577. , and Gray, J. W. (1985). Cyrometry 6,521-530. , and Gray, J. W. (1990). I n “Methods in Cell Biology” (Z.

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Flow Cytometry by Zbigniew Darzynkiewicz, J. Paul Robinson and Harry A. Crissman (Eds.)


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