By CIBA Foundation Symposium
Popular members offer entire insurance of calcium gradients, waves and oscillations in different structures. Discusses the mechanisms beginning and maintaining calcium waves and their function in mobilephone functionality. Describes stories utilizing the most recent recommendations for measuring calcium ion gradients together with chemiluminescent symptoms.
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Additional resources for Ciba Foundation Symposium 188 - Calcium Waves, Gradients and Oscillations
This compartmentalized fura-2 is not suitable for monitoring [ Ca2+] changes in the stores unless they are almost totally depleted of Ca2+. However, as originally noted by Glennon et a1 (1992), activation of InsP3-sensitive channels allows Mn2 to pass from the cytosolic compartment into the stores, where it quenches the fura-2 fluorescence. We have utilized this ability of Mn2+ to act as a Ca2+ surrogate, passing in a retrograde fashion through the InsP3-activated channels, to obtain spatially-resolved information on the Ca2+ stores.
On the 360nm signal the dye appears to be leaking out, but we’re confident that it isn’t actually leaking out in our cells. Thomas: In our fluo-3-loaded liver cells the fluo-3 is definitely pumped out, because with the intact liver we see it arrive in the bile canaliculi. We have tried injecting the liver with dextran dyes, but got rather little signal, because of heavy metal quenching, we suspect. I don’t know whether there was a progressive Subcellular organization of hepatic Ca2+ signals 37 change because we never got a signal in the first place.
Alternatively, the primary purpose of this organization may be to allow Ca2+ to flow back t o the release sites during Ca2+ reuptake following a Ca2+ spike, analogous to the partial segregation of Ca2+ release and reuptake functions in striated + FIG. 3. Vasopressin-induced quenching of compartmentalized fura-2 by Mn2+ in intact hepatocytes. Isolated hepatocytes were loaded with fura-2/AM under conditions designed to maximize compartmentalization of the dye, as described by Hajnoczky et al (1994).
Ciba Foundation Symposium 188 - Calcium Waves, Gradients and Oscillations by CIBA Foundation Symposium