Lester Packer (Eds.)'s Carotenoids Part A: Chemistry, Separation, Quantitation, and PDF

By Lester Packer (Eds.)

ISBN-10: 0121821145

ISBN-13: 9780121821142

This quantity and its spouse, quantity 214 of Methods in Enzymology, current a finished, state of the art compilation of the molecular and mobile technique wanted for pursuing examine with carotenoids

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Additional resources for Carotenoids Part A: Chemistry, Separation, Quantitation, and Antioxidation

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Physiol. B 7211, 157 (1982). 2 T. Matsuno, M. Ookubo, T. Nishizawa, and I. Shimizu, Chem. Pharm. Bull. 32, 4309 (1984). METHODS IN ENZYMOLOGY,VOL. 213 Copydght© 1992by AcademicPre~ Inc. All rightsofreproductionin any form reserved. 5 Eq) in dry DMF (45 ml) is added Pd(PPh3)2C12~° (330 mg, 3 mol%). The mixture is heated with stirring at 75 ° for 22 hr under N 2. After cooling, the reaction solution is diluted with ether and washed with 5% HC1 saturated with NaC1, saturated aqueous NaHCO3 solution, and then brine.

3] STRUCTURE AND CHARACTERIZATION OF CAROTENOIDS 27 Instruments Melting points are m e a s u r e d o n a Y a n a g i m o t o (Kyoto, J a p a n ) micromelting p o i n t apparatus. Visible a n d I R spectra are recorded o n a Shim a d z u (Kyoto, J a p a n ) U V - 2 4 0 s p e c t r o p h o t o m e t e r in ether a n d o n a Shim a d z u I R - 2 7 G s p e c t r o p h o t o m e t e r in K B r disks, respectively. M S spectra are o b t a i n e d with a Hitachi (Tokyo, Japan) M - 8 0 i n s t r u m e n t with a direct inlet system at 7 0 e V , 1 9 0 - 2 1 0 °.

M S spectra are o b t a i n e d with a Hitachi (Tokyo, Japan) M - 8 0 i n s t r u m e n t with a direct inlet system at 7 0 e V , 1 9 0 - 2 1 0 °. IH N M R (300 M H z ) spectra are recorded with a Varian (Sunnyvale, CA) X L - 3 0 0 i n s t r u m e n t in CDC13 with tetramethylsilane as an internal standard. C D spectra are m e a s u r e d o n a Jasco (Tokyo, Japan) 500-C spectropolarimeter in E P A ( e t h e r i s o p e n t a n e - e t h a n o l , 5 : 5 : 2 ) solvent at 20 °. H P L C is carried o u t o n a S h i m a d z u L C - 6 A i n s t r u m e n t with a S h i m a d z u S P D - 6 V A U V - V I S spect r o p h o t o m e t f i c detector set at 450 n m .

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Carotenoids Part A: Chemistry, Separation, Quantitation, and Antioxidation by Lester Packer (Eds.)


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