By John N. Abelson, Melvin I. Simon, John R. Sokatch, Robert Adron Harris
This quantity contains chapters at the latest innovations for measuring metabolites of branched-chain amino acids, many new enzyme assays comparable to the size of isozymes of acetohydroxacid synthase, and the purification of lately defined enzymes similar to isovaleryl-CoA dehydrogenase
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49, 591 0984). 4 In earlier publications, this compound was called l-fluoro-2,4-dinitrophenyl-L-alaninamide. Since this name does not correspond to any accepted system of nomenclature, the name consistent with IUPAC rules of organic chemical nomenclature is used in this article. We thank Dr. Kurt Loening(ChemicalAbstractsService)for assistance with this nomenclature. 5Pierce ChemicalCompany,"Previews,"p. 9. PierceChemicalCompany,Rockford,Illinois, 1985. 16 ANALYTICAL AND SYNTHETIC METHODS  with occasional shaking.
12 ANALYTICAL AND SYNTHETIC METHODS  removed by centrifugation at 10,000 g for 5 min prior to analysis of the phosphopeptides. This method is particularly useful for preparative-scale work to allow purification of sufficient amounts of peptides for sequence analysis. Alternatively, the phosphorylated protein can be precipitated by addition of ice-cold TCA to a final concentration of 10% (w/v). After standing on ice for l0 min, the resulting precipitate is collected by centrifugation at 10,000 g for 5 min.
2190. 00375. 04433. 04430. 1444. The substitution of numerical data into these equations and their solution is most conveniently accomplished with a computer program written for this purpose. Copies of a listing of such a program written in BASIC is available on request from one of us (OAM). rsonal computer, and will accept areas, sample volume, and internal standard concentrations, and calculate concentrations for the six acids. Suggested Protocol It is suggested that the procedure be set up in the following order: 1.
Branched-Chain Amino Acids by John N. Abelson, Melvin I. Simon, John R. Sokatch, Robert Adron Harris