Read e-book online Becker's world of the cell, 8th Edition PDF

By Jeff Hardin

ISBN-10: 0321716027

ISBN-13: 9780321716026

Greatly praised for its robust biochemistry assurance, Becker’s global of the telephone, 8th version, offers a transparent, up to date advent to mobile biology techniques, methods, and applications. Informed via decades of training the introductory telephone biology direction, the authors have additional new emphasis on smooth genetic/genomic/proteomic ways to telephone biology whereas utilizing transparent language to make sure that scholars understand the fabric. Becker’s global of the mobile presents available and authoritative descriptions of all significant rules, in addition to detailed medical insights into visualization and functions of cellphone biology.

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Extra info for Becker's world of the cell, 8th Edition

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Tsien for the discovery and development of GFP. An inherent limitation of fluorescence microscopy is that the viewer can focus on only a single plane of the specimen at a time, yet fluorescent light is emitted throughout the specimen, blurring the image. This problem is largely overcome by confocal scanning, which uses a laser beam to illuminate just one plane of the specimen at a time. When used with thick specimens such as whole cells, this approach gives much better resolution. In the paired fluorescence and confocal images of cheek cells at the bottom of Table 1-1, the confocal image gives much better resolution, and even allows the small bacteria in the cheek cell sample to be seen easily.

As a result, the useful magnification of the electron microscope is also much higher—up to 100,000ϫ (see Figure 1-3). Most electron microscopes have one of two basic designs: the transmission electron microscope (TEM) and the scanning electron microscope (SEM). Both are described in detail in the Appendix and images from each are shown in Figure 1-4. Transmission and scanning electron microscopes are similar because each employs a beam of electrons, but they use quite different mechanisms to form the image.

Experiments using live cells or organisms are referred to as in vivo (“in life”), often using one of a variety of popular model organisms (Box 1B). More recently, experiments using computers to test new hypotheses involv- production of genes and proteins for research, industrial, and medical uses. For studies of eukaryotic cell biology, S. cerevisiae, a unicellular fungus also known as bakers’ or brewers’ yeast, has many of the same experimental advantages. Like E. coli, yeast grows rapidly on growth media in the lab and is easily manipulated and mutagenized.

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Becker's world of the cell, 8th Edition by Jeff Hardin

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